If this share is running Linux, it doesn't matter what filesystem it really is as Samba would be the only way to access it from every PC in the house. Just cause its FAT32 doesn't automatically make it available to share since you using Linux.
Personally I would use EXT3 if this is going to be on a Linux only system, this way during a crash or the likes, Linux can easily repair the filesystem, etc. With a non-native Linux filesystem like fat32 on it, its not as easy.
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