It depends on what you want to do.
1. ALWAYS make a backup.
Btrfs includes tools that can convert an ext4 filesystem into a btrfs filesystem without needing to copy files into a btrfs filesystem.
Before you do the /boot partition, make absolutely sure that your boot system can handle it... (grub2 looks like it can:
https://www.howtoforge.com/boot-on-btrfs-with-debian
The directions are for debian, but I don't think others using grub2 will be all that different.