grep a file for any interger larger than X and assign largest value to a variable

kmkocot · 11-08-2009, 05:30 PM

Hi all,

I have a directory full of amino acid sequence files formatted like this:

Code:

>14424|LGIG|134818
MEIVTTDSVSIVETPTWKRPLELLNS
>14424|NVEC|199771
MADEGSGFSDCSSRQYLNPLQLFEEK
>14424|CAP|112268
MTTYKRPLELLEGDSLVKICAPMVRY
>14424|HROB|107587
MSVEENKFKNSMELFESKDVVKICAP
>14424|Euprymna_scolopes_pep.fas|ESCO_227709683_c_s
QKMEHAGASWVTVHGRTPEQRGEPVN

I want to write a script that will go through and count the number of occurrences of particular strings associated with the taxon names and if there are more than X sequences for a particular taxon, delete the entire file.

Here is the code I have so far using X=1 for this example:

Code:

for FileName in *.fa
do
grep -c ESCO_ $FileName >> taxon_count.txt
grep -c CAP| $FileName >> taxon_count.txt
grep -c LGIG| $FileName >> taxon_count.txt
grep -c NVEC| $FileName >> taxon_count.txt
grep -c HROB| $FileName >> taxon_count.txt


#??? somehow grep for any integer larger than 1 in taxon_count.txt and assign the largest value recovered in the variable $sequences_per_taxon

if [ "$sequences_per_taxon" -gt 1 ] ; then
printf "More than one sequence per taxon in $FileName"
mv $FileName ./rejected/
fi
done

Can anyone help me with the missing step?

Thanks!
Kevin

Tinkster · 11-08-2009, 05:41 PM

Help us :}

We're not biochemists (most people here, anyway). Which part of the
strings above is your taxon. And as you seem to be slapping all matches
into the same taxon_count.txt, how do you want to go about determining
which files to actually delete?

Cheers,
Tink

ghostdog74 · 11-08-2009, 05:56 PM

use awk for parsing files. Use arrays to store your count. Its also not clear which field is your "taxon", so i assume it is. A sample:
2nd field where line starts with ">"

Code:

awk -F"|" '/^>/{ 
 taxon[$2]++
}
END{
 for(o in  taxon){
    print o,taxon[o]
 }
}
' file

kmkocot · 11-08-2009, 08:38 PM

Thanks for the replies.

Tinkster, I forgot to include a line deleting the taxon_count.txt file after each .fa file is processed. The taxon name abbreviations for the sequences I pasted below would be LGIG, NVEC, CAP, HROB, and ESCO respectively. The problem is the 'header line' (the line containing the greater-than sign) varies in format from taxon to taxon but maybe the best thing to do is to reformat them all before processing them with these scripts.

ghostdog74, thanks for your help. My brain is fried for tonight but tomorrow I am going to try to implement your awk suggestion into the script I have now. I may have more questions.

Thanks again to both of you.

Kevin

kmkocot · 11-09-2009, 10:56 AM

The problem I ran into is that all my input files are formatted differently so instead of format each one to have the taxon abbreviation in the same field (for awk), I stuck with a grep-based approach. This greps taxon abbreviations and gets rid of input files that fewer than 6 different taxa. I know its not very pretty or versatile but it gets the job done.

Code:

for FileName in *.fa
do
grep -c NEOM_ $FileName >> $FileName\.taxon_count
grep -c WARG_ $FileName >> $FileName\.taxon_count
grep -c CNIT_ $FileName >> $FileName\.taxon_count
grep -c SVEN_ $FileName >> $FileName\.taxon_count
grep -c CAPI_ $FileName >> $FileName\.taxon_count
#... others removed for brevity
grep -c CAP\| $FileName >> $FileName\.taxon_count
grep -c LGIG\| $FileName >> $FileName\.taxon_count
grep -c NVEC\| $FileName >> $FileName\.taxon_count
grep -c HROB\| $FileName >> $FileName\.taxon_count
taxon_count=`grep -v 0 $FileName\.taxon_count | wc -l`
if [ "$taxon_count" -lt 6 ] ; then
printf "Too few taxa in file $FileName \n"
mv $FileName ./rejected_few_taxa/
fi
rm -rdf *.fa.taxon_count
done

What I am trying to do now is get rid of files that have MORE than X sequences from any one taxon. Could you guys suggest a way I could search my temporary $FileName.taxon_count file for any integer larger than X and perform an operation on that file if one is found?

Thanks again!!
Kevin

ghostdog74 · 11-09-2009, 05:26 PM

that's a slow and badly written code. there's no need to use grep so many times on each file. use grep "word1|word2" to grep multiple patterns.