It's not a matter or being root, isofilesystems are read-only and you will likely get an error to that effect if you try to save the file. I think there may be an option for rw when you mount but I've not tried that. I usually just copy the mounted directory to my /home/user directory, make the changes and use mkisofs to create a new iso.
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Or simply unmounting it would be sufficient.
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I believe you would need to generate a new iso file.