Hi,

I am using linux(Linux raghul-laptop 2.6.32-31-generic #61-Ubuntu SMP Fri Apr 8 18:24:35 UTC 2011 i686 GNU/Linux)and I just installed a BLAST standalone module in my system. I follwed the steps available here: http://www.ncbi.nlm.nih.gov/staff/ta...nix_setup.html

While running the program I get the follwoing error...

raghul@raghul-laptop:/$ /home/raghul/ncbi-blast-2.2.25+/bin/blastn -help
bash: /home/raghul/ncbi-blast-2.2.25+/bin/blastn: cannot execute binary file

Can anyone tell me what I am missing here....????

Thanks

What is:saying?

follwed and got the following messages.....

raghul@raghul-laptop:/$ file /home/raghul/ncbi-blast-2.2.25+/bin/blastn
/home/raghul/ncbi-blast-2.2.25+/bin/blastn: ELF 64-bit LSB executable, x86-64, version 1 (SYSV), dynamically linked (uses shared libs), for GNU/Linux 2.4.1, stripped
raghul@raghul-laptop:/$ file /home/raghul/ncbi-blast-2.2.25+/bin/blastn -help
Usage: file [-bcikLhnNrsvz0] [-e test] [-f namefile] [-F separator] [-m magicfiles] file...
file -C -m magicfiles
Try `file --help' for more information.

Looks like you have a 32 bit kernel and a 64 bit binary. You will have to install the 32 bit version.

I guess you picked up rightly....here is the configurtaion...

Linux raghul-laptop 2.6.32-31-generic #61-Ubuntu SMP Fri Apr 8 18:24:35 UTC 2011 i686 GNU/Linux

i686 means 32 bit right???

how could I upgrade the kernel?? is it a complicated process?

I checked and it says i have to install the kernel again...and If I do it what about my windows partition. Do I need to take a beckup...or repeat the whole process again???

I am sorry for all this question which I just blasted on you...i am new to linux...!!!

Thanks for your replies so far..witing for the same...

ciao

You can’t just upgrade the kernel, as you would have to upgrade all tools of the OS too, which means in the essence to reinstall Linux. What about installing the 32 bit version of the blastn application?

Nice Idea...

I will try the 32 bit version of BLAST...

The only hitch is ...I dont know how to uninstall previous version from the system...i tried with syneptic manager/software centre..but all in vein..so if you can tell how to uninstall the previous install version from terminal..I will proceed with installing the 32 bit BLAST version...

If you only untar the archive you can get rid of it by just removing it again:Note that will remove the complete ncbi-blast-2.2.25+ directory.