I took this info directly from a DOSemu site here.
"The simplest and safest way to initially configure DOSemu is by using a boot floppy. Create a bootable DOS floppy from your favorite DOS distribution. DOSemu supports MS-DOS Versions 3.3 through 6.22, as well as DR-DOS 6.0.
In addition to bootable DOS, the disk should have the following DOS files:
DOS mouse driver for your mouse
A simple file editor such as edit, edlin, or ted
From the dosemu subdirectory, copy the following device drivers onto the floppy:
From the command subdirectory copy the following file:
You definately need some incarnation of dos, since this isn't a true emulator, but a shell in which dos can be run.