One note of clarification... you are not burning the whole .iso file to the CD as one big file. You are creating a cd from an iso image. For example, in E-Z Cd Creator, do File - > Create CD From Image and select type .iso (.cif will be default). Then point to the .iso file on your hard disk.
It will then create the image based on the information in the .iso.
It will burn a disc that is bootable, assuming your bios supports booting from the CD rom.
This is NOT the same thing as simply writing the .iso file itself to the disc. Some burning software (e.g., IBM Record Now, HP Record Now, both made by Veritas) does not support creating CDs from iso images. This type of software is useless for your purposes here.